8-Epixanthatin is a potential colchicine binding site inhibitor isolated from Xanthium chinese Mill. 8-Epixanthatin can inhibit the activation of STAT3 , induce apoptosis , and has anti-tumor activity. In Vitro 8-Epixanthatin(2-20 μM, 12 h) inhibits DU145 cell proliferation and p-STAT3 levels of DU145 cell in a dose-dependent manner with an IC 50 value of 3.2 μM. 8-Epixanthatin can inhibit STAT3 activation and cell proliferation by inducing ROS production in DU145 cancer cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: DU145 cell Concentration: 2, 5, 10, and 20 μM Incubation Time: 12 h or 48 h Result: Reduced STAT3 phosphorylation by 90% at a concentration of 20 μM but not p-JAK2-Y1007/1008. Significantly reduced the amount of BCL-2 and BCL-xL, and induces BCL-xL PARP cleavage, the percentage of cells in G0/G1 phase decreased. In Vivo 8-Epixanthatin (i.p., 50 mg/kg, 5 days per week for 25 days) can inhibit tumor growth through STAT3 inactivation, the tumor volume of mice was reduced by 40.1%, the tumor weight was reduced by 40.0%, and the p-STAT3 level in the tumor was significantly reduced in the mouse xenograft model of DU145 cells . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid