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名称

流式细胞固定破膜剂

分子类型

生物试剂/缓冲液

英文名称

Fixation/Permeabilization Buffer

货号

F1373934-100ml

包装规格

100ml

级别

无菌过滤

浓度

10×

储存温度

2-8°C储存

运输条件

冰袋运输

稳定性与储存

Store at 2-8℃ long term (12 months).

产品介绍

固定剂、破膜剂用于细胞内细胞因子染色前对细胞膜的处理。固定剂起稳定细胞膜、保持细胞膜表面抗体与抗原结合的作用；破膜剂使流动的、完整的细胞膜产生小孔以利于抗体进入细胞。用流式细胞仪检测细胞内抗原及DNA时，对待测细胞进行固定、破膜处理后，加入相应抗体或检测试剂，孵育一定时间即可上机分析。使用说明 1、至少收集1×10⁶ 待测细胞，1500rpm离心5min，弃上清。 2、加入1000μl固定破膜液。 3、处理15min。 4、1500 rpm离心5min。 5、弃上清，加入PBS洗一次，1500rpm离心5min。 6、弃上清，按检测指标要求，进行后期染色并上机。注： 1、如果有膜蛋白，请在步骤2前，先染膜蛋白，再做步骤2的固定破膜； 2、该产品对胞浆抗原检测效果较好，胞核指标，建议用货号 [F1373935] 流式核膜破膜剂。注意事项： 1、为了您的安全和健康，请穿实验服并戴一次性手套操作。 2、粉末溶解前请先短暂离心，以保证产品全在管底。 3、请勿吸入、吞咽或者直接接触皮肤和眼睛。 4、本产品仅用于科研用途，禁止用于人身上。

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After Sales Services

Please check the quantity, appearance and product information in time when you receive the goods. In case of damage, wrong delivery, abnormal quality and other problems, please feel free to contact us.

Service Hotline:400-6226-992 ，We will handle it for you as soon as possible, thank you for your support and trust.！