Fmoc-Trp-BO二PY

芴甲氧苄啶

Trp-BODIPY

Fmoc-Trp-BODIPY

F463570-100mg

100mg

≥95%

应用 该化合物是一种 fmoc 标记的色氨酸，通过无间隔的 C-C 键连接到 BODIPY 染料上，可用于固相多肽合成(SPPS)。在 Mendive-Tapia 等人的研究中，测试了通过替换一个或两个残基，将荧光氨基酸掺入具有两个色氨酸的抗真菌肽中。这三种肽显示出增强的荧光，同时增强了环境的疏水性。肽还保持与烟曲霉的结合，并且可以通过荧光成像检测。同一篇文章显示，含有单一荧光氨基酸的肽的环状形式比线性肽更耐蛋白水解，并且仍适用于成像。该氨基酸可以在 SPPS 的标准条件下掺入，并且还可以耐受 1％ 三氟乙酸的轻度酸性条件，允许使用酸不稳定的固体载体。该氨基酸应能够对肽进行荧光检测，用于各种应用。 Description This compound is an Fmoc-labeled amino acid of tryptophan attached to a BODIPY dye through a spacer-free C-C linkage that can be used in solid phase peptide synthesis (SPPS). Research presented in Mendive-Tapia et al. tested the incorporation of the fluorescent amino acid into an anti-fungal peptide with two tryptophans by replacing either one or both of the residues. The three peptides showed enhanced fluorescence with increasing hydrophobicity of the environment. The peptides also maintained binding toAspergillus fumigatusand could be detected by fluorescence imaging. That same publication showed that a cyclic version of the peptide containing a single fluorescent amino acid was more resistant to proteolysis than the linear peptide and still useful for imaging. The amino acid can be incorporated during standard conditions for SPPS and can also tolerate mildly acidic conditions of 1% trifluoroacetic acid allowing use of acid-labile solid supports. This amino acid should enable fluorescence detection of peptides for use in a variety of applications.