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Product details

名称

FastSYBR Mixture

别名

FastSYBR 混合物

英文名称

FastSYBR Mixture

货号

F665884-5ml

包装规格

5ml

浓度

5 ml

储存温度

-20°C储存

运输条件

超低温冰袋运输

产品介绍

FastSYBR Mixture是专用于染料法(SYBR GreenⅠ)实时荧光定量PCR的预混体系，浓度为2×,包含Fast Taq DNA Polymerase、PCR Buffer、dNTPs、SYBR Green I 荧光染料和Mg2+，操作简单方便。主要用于基因组DNA靶序列和RNA反转录后的 cDNA靶序列检测。本品所含荧光染料SYBR Green I可以与所有的双链DNA相结合，使该产品可用于不同靶序列的检测而不需合成特异性标记探针。本品含有的Fast Taq DNA Polymerase能有效减少在常温条件下由引物和模板非特异性结合或引物二聚体而产生的非特异性扩增，酶的激活仅需在95℃孵育20s。整个PCR反应过程比普通反应可节省约40分钟，大大缩短了PCR的反应时间。独特的PCR缓冲体系与热启动酶的组合，有效抑制了非特异产物的产生，并显著提高PCR的扩增效率。该产品应用范围广，适用于普通和快速定量PCR程序。 ROX染料用于校正定量PCR仪孔与孔之间产生的荧光信号误差，一般用于ABI、 Stratagene等公司的Real Time PCR 扩增仪。不同仪器的激发光学系统有所不同，因此ROX染料的浓度必须与相应的荧光定量PCR仪相匹配。不需要ROX校正的仪器： Roche LightCycler 480，Roche LightCyler 96，Bio-rad iCyler iQ，iQ5，CFX96等。需要Low ROX校正的仪器： ABI Prism7500/7500 Fast，QuantStudio® 3 System，QuantStudio® 5 System， QuantStudio® 6 Flex System，QuantStudio® 7 Flex System，ViiA 7 system， Stratagene Mx3000/Mx3005P，Corbett Rotor Gene 3000等。需要High ROX校正的仪器： ABI Prism7000/7300/7700/7900，Eppendorf，ABI Step One/Step One Plus等。 F665884

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