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Product details

名称

Fura-2 AM (钙离子荧光探针)

英文名称

Fura-2 AM

货号

F746140-50μl

包装规格

50μL

浓度

2mM

储存温度

避光,-20°C储存

运输条件

超低温冰袋运输

稳定性与储存

-20℃避光保存，6个月有效。

产品介绍

Fura-2 AM，也称Fura-2 pentakis ester，是最常用的检测细胞内钙离子浓度的荧光探针之一。分子式为C44H47N3O24，分子量为1001.9。本Fura-2 AM(钙离子荧光探针)是配制于无水DMSO(anhydrous DMSO)中的储存液，浓度为2mM。Fura-2 AM是一种可以穿透细胞膜的荧光染料。Fura-2 AM的荧光比较弱，最大激发波长为369nm，最大发射波长为478nm，并且其荧光不会随钙离子浓度改变而改变。Fura-2AM进入细胞后可以被细胞内的酯酶剪切形成Fura-2，从而被滞留在细胞内。Fura-2可以和钙离子结合，结合钙离子后在330-350nm激发光下可以产生较强的荧光，而在380nm激发光下则会导致荧光减弱。这样就可以使用340nm和380nm这两个荧光的比值来检测细胞内的钙离子浓度，可以消除不同细胞样品间荧光探针装载效率的差异，荧光探针的渗漏，细胞厚度差异等一些误差因素。Fura-2和钙离子结合后，最大激发波长为335nm(最大激发波长随离子浓度的的不同而有所不同)，最大发射波长为505nm。实际检测时推荐使用的激发波长为340nm，发射波长为510nm。如果做双波长检测，则推荐使用的激发波长为340nm和380nm。Fura-2的激发光谱参考下图，不同曲线表示不同钙离子浓度时的激发光谱。用于细胞内钙离子检测时，Fura-2 AM的常用浓度为0.5-5μM。通常用含有0.5-5μM的Fura-2 AM的适当溶液和细胞一起在4-37℃孵育15-60分钟，即可完成荧光探针的装载。注意事项：本Fura-2AM在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内，可以20-25℃水浴温育片刻至全部融解后使用。荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。 Precautions ： This product will solidify and adhere to the interior wall of the vial at low temperatures, such as 4℃. Prior to use, it should be defrosted by incubation in a 20-25℃ water bath. Please store this product away from light to minimize fluorescence quenching.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

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