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名称

无花果蛋白酶来源于无花果树乳胶

货号

F755315-200U

包装规格

200U

浓度

lyophilized powder

产品介绍

来自无花果树乳胶的无花果蛋白酶已被用于: 肌腱胶原蛋白的蛋白水解石莼属绿藻的消化研究其激活蛋白酶激活受体 2和 4 诱发皮肤瘙痒的作用应用：消光系数： E1% = 21.0 (280 nm)pI: 9.0 Extinction Coefficient: E 1% = 21.0 (280 nm) pI: 9.0 Application: Ficin from fig tree latex has been used: in the proteolysis of tendon collagen in the digestion of Chlorophyta Ulva rigida to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin

生化机理

无花果蛋白酶可以有效地分解葡萄球菌生物膜，从而使其接触抗生素。无花果蛋白酶（Ficin）被归类为硫醇蛋白酶。它的活性位点上含有单个反应性半胱氨酸。其活性位点的氨基酸同源性与木瓜蛋白酶相似。无花果蛋白酶将切割Gly、Ser、Thr、Met、Lys、Arg、Tyr、Ala、Asn和Val羧基侧的蛋白质。据报道，显色底物pGlu-Phe-Leu-p-硝基苯胺的 K m 为0.43 mM。无花果蛋白酶可被碘乙酰胺、碘乙酸、N-乙基马来酰亚胺、氯化汞、DFP（二异丙基氟磷酸酯）、TLCK（Na-对甲苯磺酰基-赖氨酸氯甲基酮）和TPCK（N-甲苯磺酰基-L-苯丙氨酸氯甲基酮）抑制。无花果蛋白酶可用于以高得率产生小鼠IgG1的F (ab′) 2 片段。

Merck Index

4077

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

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