Goldview核酸染料

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Goldview Nucleic Acid Dyes

G273088-1ml

1ml

10000× in DMSO

2-8°C储存,避光,干燥

冰袋运输

对光和湿度敏感

本品用DMSO溶解，因DMSO的熔点是18.5℃，使用前请放置到室温充分溶解。 产品介绍 Goldview 是一种绿色条带的核酸染料，但是可能与EB一样对细胞有致突变性，采用琼脂糖电泳检测DNA时，Goldview与核酸结合后能产生很强的荧光信号，其灵敏度与EB相当，使用方法与之完全相同。在紫外灯下双链DNA呈现绿色荧光，而单链DNA呈红色荧光。 产品参数：Ex(nm)：492，Em(nm)：526 使用方法 1. 将100mL琼脂糖凝胶溶液（浓度一般为0.8%~2%）在微波炉中融化。 2. 加入5μL Goldview，轻轻摇匀，避免产生气泡。 3. 冷却至不烫手时倒胶，待琼脂糖凝胶完全凝固后上样电泳。 4. 电泳完毕在紫外灯或可见光下观察。若使用凝胶成像系统照相，通过调节光圈、曝光时间，选择合适的滤光片，可得到成像清晰、背景较低的照片。若使用数码相机照相记录，则关闭相机的闪光灯，放在自动档即可。 注意事项 1. Goldview 是一种绿色条带的核酸染料，但是可能与EB一样对细胞有致突变性, 操作时应和EB一样注意防护。 2. 加入Goldview的琼脂糖凝胶反复融化可能会对核酸检测的灵敏度产生一定影响，但不明显。 3. Goldview在pH 3.6~7.0之间能更好的与核酸结合，因此电泳时最好使用新鲜的电泳缓冲液。 4. 由于Goldview产生绿色荧光，因此不适合于用一般单色胶卷拍照，可以使用凝胶成像系统保存图像。 5. 含有Goldview的凝胶不适合于进行凝胶回收试验。要进行回收试验，请使用SYBR Green I核酸染料。 6. Goldview特别适合于大片段DNA的检测(大于1kb的片段，检测灵敏度与EB相当)；当DNA片段小于1kb时，检测灵敏度低于EB，特别是低于500bp的片段，Goldview可能亮度很弱或者检测不到，如要检测小片段的DNA，请选用SYBR Green I核酸染料。 7.胶厚度不宜超过0.5cm，胶太厚会影响检测的灵敏度。 8. 由于Goldview溶液酸性较强，因此对皮肤、眼睛会有一定的刺激，操作时应戴手套。 This product is dissolved in DMSO. Because the melting point of DMSO is 18.5℃, please put it to room temperature to dissolve fully before use. Product description Goldview is a nucleic acid dye with a green band, but it may be mutagenic to cells like EB. When DNA is detected by agarose electrophoresis, Goldview can produce a strong fluorescent signal after binding to nucleic acid. Its sensitivity is equivalent to EB. The method is exactly the same. Double-stranded DNA exhibits green fluorescence under ultraviolet light, while single-stranded DNA exhibits red fluorescence. Product parameters: Ex (nm): 492, Em (nm): 526 Instructions 1. Melt 100mL agarose gel solution (concentration is generally 0.8%~2%) in a microwave oven. 2. Add 5μL Goldview and shake gently to avoid air bubbles. 3. Pour the gel when it is not hot, and load the sample for electrophoresis after the agarose gel has completely solidified. 4. After electrophoresis, observe under ultraviolet light or visible light. If you use a gel imaging system to take pictures, by adjusting the aperture, exposure time, and selecting the appropriate filter, you can get a clear image with a low background. If you use a digital camera to take photos, turn off the camera's flash and put it in automatic mode. Precautions 1. Goldview is a nucleic acid dye with a green band, but it may be as mutagenic to cells as EB. You should pay attention to the same protection as EB during operation. 2. Repeated melting of the agarose gel added to Goldview may have a certain impact on the sensitivity of nucleic acid detection, but it is not obvious. 3. Goldview can better bind to nucleic acids at pH 3.6~7.0, so it is best to use fresh running buffer during electrophoresis. 4. Since Goldview produces green fluorescence, it is not suitable for taking pictures with general monochromatic film. You can use a gel imaging system to save images. 5. Gels containing Goldview are not suitable for gel recovery tests. For the recovery test, please use SYBR Green I nucleic acid dye. 6. Goldview is particularly suitable for the detection of large fragments of DNA (fragments greater than 1kb, the detection sensitivity is equivalent to EB); when the DNA fragment is less than 1kb, the detection sensitivity is lower than EB, especially for fragments less than 500bp, Goldview may have weak brightness Or it cannot be detected. If you want to detect small fragments of DNA, please use SYBR Green I nucleic acid dye. 7. The thickness of the glue should not exceed 0.5cm, too thick glue will affect the sensitivity of detection. 8. Since the Goldview solution is relatively acidic, it will irritate the skin and eyes to a certain extent. Wear gloves when operating.