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Product details
名称
GoldStar Probe Mixture
别名
GoldStar 探针混合物
英文名称
GoldStar Probe Mixture
货号
G665832-5ml
包装规格
5ml
浓度
5 ml
储存温度
-20°C储存,避免反复冻融
运输条件
超低温冰袋运输
产品介绍
GoldStar Probe Mixture是专用于探针法(TaqMan,Molecular Beacon等)实时 荧光定量PCR的预混体系,浓度为2×,包含GoldStar Taq DNA Polymerase、PCR Buffer、dNTPs和Mg2+,操作简单方便。主要用于基因组DNA靶序列和RNA反转录后 cDNA靶序列检测,如基因表达分析,拷贝数分析,SNP基因型分析等,适用于不同类 型探针法荧光定量。本品含有的GoldStar Taq DNA Polymerase是一种经化学修饰的、 全新高效热启动酶,在常温下没有聚合酶活性,有效避免了在常温条件下由引物和模 板非特异性结合或引物二聚体而产生的非特异性扩增,酶的激活须在95℃下孵育10分 钟。独特的PCR缓冲体系与热启动酶的组合,显著提高了PCR的扩增效率,荧光信号 更强,灵敏度更高,可以检测单拷贝的模板。使用本产品可以得到更广的线性范围, 对目的基因定量更准确。适用于无需ROX作为校正染料的所有荧光定量PCR仪。 ROX染料用于校正定量PCR仪孔与孔之间产生的荧光信号误差,一般用于ABI、 Stratagene等公司的Real Time PCR 扩增仪。不同仪器的激发光学系统有所不同,因 此ROX染料的浓度必须与相应的荧光定量PCR仪相匹配。 不需要ROX校正的仪器(CW0932): Roche LightCycler 480,Roche LightCyler 96,Bio-rad iCyler iQ,iQ5,CFX96等。 需要Low ROX校正的仪器(CW2625): ABI Prism7500/7500 Fast,QuantStudio® 3 System,QuantStudio® 5 System, QuantStudio® 6 Flex System,QuantStudio® 7 Flex System,ViiA 7 system, Stratagene Mx3000/Mx3005P,Corbett Rotor Gene 3000等。 需要High ROX校正的仪器(CW2626): ABI Prism7000/7300/7700/7900,Eppendorf,ABI Step One/Step One Plus等。 G665832
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「No Dangerous Attributes」
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GoldStar Probe Mixture 5ml

item number:G665832-5ml
Product model:5ml
level: 5ml
Lead Time:30days
sold 539 Items
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5ml

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