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名称
β-葡萄糖苷酶
英文别名
beta-glucosidase | amygdalase | beta-D-glucoside glucohydrolase | cellobiase | gentobiase | β-D-Glucoside glucohydrolase | b-D-Glucosidase | b-D-Glucoside glycohydrolase | EC 3.2.1.21 | gentiobiase | emulsin | elaterase | aryl-beta-glucosidase | beta-D-gl
货号
G755171-100U
包装规格
100U
级别
生物活性
浓度
≥10U/mg powder; 10-60 U/mg protein
产品介绍
β- 葡萄糖苷酶是一种葡萄糖苷酶,作用于连接两个葡萄糖分子或葡萄糖取代分子(即二糖纤维二糖)的 β1->4 键。它是纤维素酶中的一种,而纤维素酶是参与纤维素及相关多糖分解的酶类;更具体地说,它是一种外切纤维素酶,对多种 β-D - 糖苷底物具有特异性。它能催化 β-D - 葡萄糖苷中末端非还原残基的水解,释放出葡萄糖。 应用 在临床分析中,当该酶与 α- 葡萄糖苷酶联合使用时,可用于碳水化合物的结构研究以及 α- 淀粉酶的酶法测定。 产品信息 来源:甜杏仁 外观:浅黄色无定形粉末,冻干品 形态:冻干粉末 分子量:约 110 kDa 活性:≥10U/mg 固体(含约 50% 的牛血清白蛋白) 污染物:α- 淀粉酶<5.0×10⁻⁴% 等电点:7.3 pH 稳定性:pH 6.0-9.0(25°C,64 小时) 最适 pH:5.5 热稳定性:50°C 以下稳定(pH 7.3,1 小时) 最适温度:50-55°C 米氏常数:2.8×10⁻³M(对硝基苯基 -β-D - 吡喃葡萄糖苷)、3.3×10⁻³M(2,4 - 二氯苯基 -β-D - 吡喃葡萄糖苷) 结构:每摩尔酶含 2 个亚基 稳定剂:牛血清白蛋白(BSA)、谷胱甘肽(还原型) 稳定性:在 - 20℃下至少稳定 6 个月(在 5℃下存放 6 个月,活性可能下降约 10%) Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking twoglucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is oneof the cellulases, enzymes involved in the decomposition of cellulose and relatedpolysaccharides; more specifically, an exocellulase with specificity for a variety ofbeta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducingresidues in beta-D-glucosides with release of glucose. Applications This enzyme is useful for structural investigations of carbohydrates and for theenzymatic determination of α-amylase when coupled with α-glucosidase in clinicalanalysis. Product Information Source: Sweet almond Appearance: Light yellow amorphous powder, lyophilized Form: Freeze dried powder Molecular Weight:approx. 110 kDa Activity: 10U/mg-solid or more (containing approx. 50% of BSA) Contaminants: α-Amylase Isoelectric point: 7.3 pH Stability: pH 6.0-9.0 (25°C, 64hr) Optimum pH: 5.5 Thermal stability: below 50°C (pH 7.3, 1hr) Optimum temperature: 50-55°C Michaelis Constant: 2.8×10⁻³M (p-Nitrophenyl-ß-D-glucopyranoside), 3.3×10⁻³M (2,4-Dichlorophenyl-ß-D-glucopyranoside) Structure: 2 subunits per mol of enzyme Stabilizers: Bovine serum albumin (BSA), glutathione (reduced) Stability: Stable at-20°C for at least 6 months (A decrease in activity of ca. 10% may occur at5°C within 6 months)
生物活性
≥10U/mg powder; 10-60 U/mg protein
来源
天然
预测分子量
~110 kDa
种属
杏仁(Almond)
Safety
「No Dangerous Attributes」
Related
「No upstream and downstream information yet」
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Research chemical

Research chemical; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number:G755171-100U
Product model:100U
level: biological activity
Lead Time:30days
sold 862 Items
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