β-葡萄糖苷酶

beta-glucosidase | amygdalase | beta-D-glucoside glucohydrolase | cellobiase | gentobiase | β-D-Glucoside glucohydrolase | b-D-Glucosidase | b-D-Glucoside glycohydrolase

G755204-250mg

250mg

生物活性

≥4 U/mg powder

催化糖苷的水解反应。苦杏仁酶（Emulsin）最初指的是甜杏仁的提取物，至今仍是 β- 葡萄糖苷酶的历史性原型。目前已发现该酶的多种其他来源。苦杏仁酶还具有显著的 D - 半乳糖苷酶活性，关于其是否为一种酶具有两种活性存在争议，因为抑制剂对两种活性的影响并不平行，这表明可能存在两种独立的酶。此外，有报道称杏仁苦杏仁酶中还含有 D - 甘露糖苷酶。 形态：浅黄色至黄棕色粉末。 溶解度：在水中呈无色至浅黄色，澄清至微浊。 溶解度浓度：1.90-2.10mg/ml。 测定方法：β- 葡萄糖苷酶水解水杨苷生成水杨醇和 β-D - 葡萄糖，通过己糖激酶 / 葡萄糖 - 6 - 磷酸脱氢酶系统测定葡萄糖的生成速率。在特定条件下（37°C、pH 5.0），1 个单位的酶活力定义为每分钟释放 1 微摩尔葡萄糖的酶量。 特异性：据报道，该酶的底物包括 β- 葡萄糖苷和 β- 半乳糖苷。 抑制剂：氯化汞（HgCl₂）、其他重金属离子、巯基结合化合物以及多元醇。 重要声明：本产品仅供研究用途，不得用于人类治疗或诊断应用。 毒性与危害： 所有产品仅限经过实验室操作培训的专业人员处理。 Catalyzes the hydrolysis of glucosides. Emulsin originally was the term applied to the extract of sweet almonds. It remains as the historical prototype of β-glucosidase. Many other sources of the enzyme have been described. Emulsin also shows significant D-galactosidase activity and there is some question as to whether or not it is one enzyme with two activities, because non-parallel response to inhibitors points to two separate enzymes. D-mannosidase has also been reported in almond emulsin. Form : Supplied as a light yellow to yellow tan powder. Solubility : Colorless to light yellow, clear to slightly hazy in water. Solubility Concentration :1.90-2.10mg/ml Assay Method : Salicin is hydrolyzed by b-glucosidase to yield saligenen and b-D-glucose. The rate of formation of glucose is measured in a hexokinase/glucose-6-phosphate dehydrogenase system. One unit releases one micromole of glucose per minute at 37°C and pH 5.0 under the specified conditions. Specificity : b-glucosides and b-galactosides are reportedly substrates for the enzyme. Inhibitors : HgCl₂, other heavy metal ions, sulfhydryl-binding compounds and polyols. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Toxicity and Hazards : All products should be handled by qualified personnel only, trained in laboratory procedures.

β-葡萄糖苷酶可参与β-D-糖苷中连接碳水化合物残基的β-糖苷键的水解。它们可将内切和外切葡聚糖酶产生的纤维二糖和纤维寡糖转化为葡萄糖。

≥4 U/mg powder

天然

~135000

杏仁(Almond)