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Product details
名称
Human BDNF ELISA Kit
分子类型
试剂盒
英文名称
Human BDNF ELISA Kit
货号
H1128695-96T
包装规格
96T
级别
生物活性
储存温度
2-8°C储存
运输条件
冰袋运输
稳定性与储存
2-8℃储存(6个月)。
产品介绍
脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)是1982年Barde等首先在猪脑中发现的一种具有神经营养作用的蛋白质。脑源性神经营养因子及其受体在神经系统广泛表达。一种小分子二聚体蛋白质BDNF结构、分布及信号转导BDNF分子单体是由119个氨基酸残基组成的分泌型成熟多肽,蛋白等电点为9.99,相对分子质量为3.5×103,主要由β折叠和无规则卷曲二级结构组成,含有3个二硫键,为一种碱性蛋白质。BDNF分布在中枢神经系统、周围神经系统、内分泌系统、骨和软骨组织等广泛区域内,但主要是在中枢神经系统内表达,其中海马和皮质的含量最高。 一、检测原理: 阿拉丁生产的ELISA试剂盒采用“夹心法”:将捕获抗体包被于酶标板上,捕获样品及标准品中的靶蛋白,生物素化的检测抗体与靶蛋白结合,SABC复合物与生物素化检测抗体结合,形成免疫复合物,加入TMB显色液后,若反应孔中有靶蛋白则显蓝色,加入终止液变黄色,检测过程中未结合的成分均被洗去,用酶标仪在450nm处测OD值,靶蛋白浓度与OD值之间呈正比,通过绘制标准曲线计算出标本中靶蛋白的浓度,从而对检测样品进行定性或相对定量分析。 二、试剂盒组分: H1128695
Safety
「No Dangerous Attributes」
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Research chemical

Research chemical; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number:H1128695-96T
Product model:96T
level: biological activity
Lead Time:30days
sold 329 Items
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96T

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