人外周血淋巴细胞分离液(FICOLL配制)

H598316-200ml

200ml

主要用于人外周血淋巴细胞的分离 注意事项： 1. 无菌条件下操作，密封后置常温保存。 2. 全过程样本、试剂及实验环境均需在 20 ± 2℃的条件下进行。为获得最佳的实验结果，最好在取血 2 h 内进行实验，血液存放时间越长，细胞分离效果越差。血液放置超过 6h 后分离效果更差甚至不能达到分离目的。 3. 本实验最好不要使用高聚合材质（如聚苯乙烯）的塑料制品，应使用无静电、低静电离心管及未经碱处理过后的玻璃制品，因为静电作用将导致细胞贴壁、碱处理的玻璃表面会变成毛面，影响细胞分离效果。 4. 吸取过多的淋巴细胞层及分离液层会导致分离液交界处的粒细胞被吸出从而使混杂的粒细胞数量增加。 5. 吸取过多的淋巴细胞层上层溶液会导致血浆蛋白及血小板混杂。 6. 当血液样本粘度过高需稀释时，最优稀释方法：将血液与样本稀释液（产品货号：H9044）1:1 稀释混匀备用。注：不当的稀释方法会降低细胞得率及活性。如血液样本经过稀释则分离过程中需适当降低离心力和离心时间。 7. 因血液样本的个体差异及血液粘度不同，需调整离心力及离心时间，离心力最大不超过 1000 g。 应用范围： 淋巴细胞分离 Product introduction： It is mainly used for the isolation of human peripheral blood lymphocytes Matters needing attention： 1. operate under sterile conditions and store at room temperature after sealing. 2. samples, reagents and experimental environment in the whole process shall be carried out at 20 ± 2 ℃. In order to obtain the best experimental results, it is best to carry out the experiment within 2 h after taking blood. The longer the blood is stored, the worse the cell separation effect is. After the blood is placed for more than 6h, the separation effect is worse or even can not achieve the purpose of separation. 3. in this experiment, it is better not to use plastic products with high polymerization materials (such as polystyrene), but use non-static, low static ionization heart tubes and glass products without alkali treatment, because the electrostatic effect will lead to cell adhesion, and the surface of alkali treated glass will become rough, which will affect the effect of cell separation. 4. aspirating too many lymphocyte layers and separation liquid layers will cause the granulocytes at the junction of separation liquid to be aspirated, thus increasing the number of mixed granulocytes. 5. absorbing too much upper layer solution of lymphocyte layer will lead to the mixing of plasma proteins and platelets. 6. when the blood sample viscosity is too high and needs to be diluted, the optimal dilution method is: dilute and mix the blood and sample diluent (product No.: h9044) 1:1 for standby. Note: improper dilution method will reduce cell yield and activity. If the blood sample is diluted, the centrifugal force and centrifugation time should be appropriately reduced during the separation process. 7. due to the individual differences of blood samples and different blood viscosity, the centrifugal force and centrifugal time should be adjusted. The maximum centrifugal force should not exceed 1000 g. Scope of application： Lymphocyte isolation