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名称
免疫染色洗涤液 (10×)
分子类型
生物试剂/缓冲液
别名
洗涤液
英文别名
Wash Buffer
英文名称
Immunol Staining Wash Buffer (10×)
货号
I752112-10×100ml
包装规格
10×100ml
级别
适用于免疫荧光(IF)
浓度
10×
储存温度
-20°C储存
运输条件
超低温冰袋运输
稳定性与储存
-20℃储存(12个月)。
产品介绍
免疫染色洗涤液可以用于免疫荧光(Immunofluorescence, IF)、免疫组化(Immunohistochemistry, IHC)、免疫细胞化学(Immunocytochemistry, ICC)和酪酰胺荧光多标(Multiplex immunohistochemical,mIHC)等免疫染色时固定、一抗或二抗孵育后的洗涤。 本产品中含有适量Triton X-100,可以通透细胞膜,经本免疫染色洗涤液洗涤的细胞或组织样品,更易于检测细胞内的目的蛋白,同时适当洗涤可以降低染色背景,增强信噪比。 本产品适用于石蜡切片,也可以用于冰冻切片、细胞等其它样品。 操作步骤 1.取100ml免疫染色洗涤液加入去离子水或蒸馏水混匀并定容到1L后即为免疫染色洗涤液(1×),用于免疫染色的洗涤。免疫染色洗涤液(1×)建议现配先用,未使用完的可短期内4ºC保存,如发现洗涤液出现浑浊、沉淀等异常现象,宜废弃重新配制。 2.在固定、一抗或二抗孵育结束后,加入适量免疫染色洗涤液,使之能浸没样品,在摇床上缓慢摇动洗涤5min后更换新的洗涤液洗涤,共洗涤3次,每次5min,然后进行后续的操作。 3.如果摇动容易导致样品脱落,可以静止浸泡洗涤。在静止浸泡洗涤的情况下,需适当延长洗涤时间至10-15min/次,增加洗涤次数至4-5次。 注意事项: 1.本免疫染色洗涤液经过滤除菌处理,使用过程中应尽量避免细菌污染。一旦启用后建议在2个月内用完。 2.本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。 3.本产品不含剧毒,但为了您的安全和健康,请穿实验服并戴一次性手套操作。 物理外观:Liquid 是否无菌:否
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Research chemical 100ml

Research chemical 100ml; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 100ml.

item number:I752112-10×100ml
Product model:10×100ml
level: 100ml
Lead Time:30days
sold 308 Items
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10×100ml

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