INT/BCIP 储备液

I755389-3ml

3ml

solution, pkg of 3 mL

INT/BCIP 储备液用于印迹方案中碱性磷酸酶 (AP) 的灵敏检测，包括： Southern印迹蛋白质印迹免疫组织化学和免疫细胞化学其已用于原位 杂交显色 。应用： BCIP（5-溴-4-氯-3-吲哚基磷酸盐）是一种 AP（碱性磷酸酶）-底物，其脱磷酸后进一步反应生成深蓝色靛蓝染料作为氧化产物。INT（碘硝基四唑）是产生红色染料的氧化剂。反应产物呈红棕色，不溶于水。 BCIP (5-bromo-4-chloro-3-indoyl phosphate) is the AP (alkaline phosphatase)-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product. INT (iodonitrotetrazolium) is the oxidant producing a red dye. The reaction product has a reddish-brown color and is insoluble in water. Application: INT/BCIP Stock Solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, that includes: Southern blot Western blot immunohistochemistry and immunocytochemistry It has been used for colorogenic in situ hybridization.