重组人胰岛素

I755826-250mg

250mg

BioReagent

dry powder, for research or for further manufacturing use

无血清培养基补充剂（如胰岛素）对于常用哺乳动物细胞系的长期生长至关重要。当培养基中缺少胰岛素时，细胞形态和生长速度可能会受到干扰。重组人胰岛素已用在培养基中的间充质干细胞的成脂诱导。 它已被用作气道上皮细胞的细胞培养基中的补充剂。 它已用于细胞分化、GLUT4（4 型葡萄糖转运蛋白）易位测定的培养基中，并通过表面等离振子共振研究胰岛素与胰岛素受体的相互作用。 重组人胰岛素（rHu）在功能和结构上与天然人序列相同。由两个多肽链组成的激素，rHu 胰岛素的 A 链（21 个氨基酸）和 B 链（30 个氨基酸）通过半胱氨酸残基之间的二硫键共价连接。这种经基因工程改造的人胰岛素是全序列分子，通过重组 DNA 技术在面包酵母中生产。 应用： 无血清培养基补充剂（如胰岛素）对于常用哺乳动物细胞系的长期生长至关重要。当培养基中缺少胰岛素时，细胞形态和生长速度可能会受到干扰。 重组人胰岛素已用在培养基中的间充质干细胞的成脂诱导。 [1] 它已被用作气道上皮细胞的细胞培养基中的补充剂。 [2] 它已用于细胞分化、GLUT4（4 型葡萄糖转运蛋白）易位测定的培养基中，并通过表面等离振子共振研究胰岛素与胰岛素受体的相互作用 Insulin, recombinant human (rHu) is identical in function and structure to the native human sequence. A hormone consisting of two polypeptide chains, rHu Insulin′s A-chain (21 amino acids) and B-chain (30 amino acids) are covalently linked by disulfide bonds between cysteine residues. This genetically engineered human insulin is a full sequence molecule producted by recombinant DNA technology in baker′s yeast. Application: Serum-free medium supplements such as insulin are essential for long-term growth of commonly used mammalian cell lines. When insulin is absent from media, cell may exhibit disturbances in morphology and growth rate. Recombinant Human Insulin has been used in the media for adipogenic induction of mesenchymal stem cells. It has been used as a supplement in the cell culture media of airway epithelial cells. It has been used in the culture media for differentiation of cells, GLUT4 (glucose transporter type 4) translocation assay and to study interaction of insulin with insulin receptor via surface plasmon resonance.

由胰岛β-细胞所产生的二链多肽激素。其分子量为~5800 Da。α链和β链是由两个链间二硫键所连接的。α链含有一个链间二硫键。胰岛素可调节细胞对葡萄糖、氨基酸和脂肪酸的摄取、利用和储存，并抑制糖原、蛋白质和脂肪的分解。