JNJ-1013 is a potent and selective IRAK1 degrader with an IC 50 s of 72, 443, 1071 nM for IRAK1 , IRAK4 , VHL FP respectively. JNJ-1013 induces Apoptosis and increases the expression of cleavaged PARP . JNJ-1013 decreases the expression IRAK1 , p-IKBα, pSTAT3(Tyr705) In Vitro JNJ-1013 (Degrader-3; 1.5-10000 nM; 24 h) dose-dependently degrades IRAK1 protein with an DC 50 value of 3 nM.. JNJ-1013 (1 µM) decreased the expression of MG-132 stiyumed IRAK1 in a dose-dependent manner. JNJ-1013 (0.01, 0.03, 0.1, 0.3, 1 µM; 24 h) decreases the expression IRAK1, p-IKBα, pSTAT3(Tyr705) and increases the expression of cleavaged PARP. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: HEK293T cells Concentration: 1.5-10000 nM Incubation Time: 24 h Result: Decreased the expression of IRAK1 in a dose-dependent manner. Form:Solid IC50& Target:IRAK1 72 nM (IC 50 ) IRAK4 443 nM (IC 50 ) VHL FP 1071 nM (IC 50 )