J14

J14

J651908-5mg

5mg

≥99%

-20°C储存

超低温冰袋运输

J14 is a reversible sulfiredoxin inhibitor with an IC 50 of 8.1 μM. J14 induces oxidative stress (intracellular ROS accumulation) by inhibiting sulfiredoxin , leading to cytotoxicity and cancer cell death In Vitro J14 (0-100 μM; 0-96 hours; A549 cells) treatment inhibits the growth of A549 cells in a concentration- and a time- dependent manner, and its half inhibitory concentration for the growth of A549 cells was 15.7 μM. ?\nJ14 (20 μM; 48-72 hours; A549 cells) treatment causes not only the release of cytochrome c into the cytosol, but also the activation of caspase-3 and caspase-9. J14 induces oxidative damage to mitochondria, resulting in caspase-mediated apoptosis. ?\nJ14 treatment significantly increases the accumulation of sulfinic peroxiredoxins and intracellular ROS. Excess accumulation of intracellular ROS causes oxidative damage, leading to cell death. J14 significantly induces cell death in A549 cells in a time-dependent manner, resulting in approximately 40% cell death in 96 hours. ?\nJ14 induces oxidative mitochondrial damage and apoptosis. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: A549 cells Concentration: 0-100 μM Incubation Time: 0 hour, 24 hours, 48 hours, 72 hours, 96 hours Result: Inhibited the growth of A549 cells in a concentration- and a time- dependent manner. Western Blot AnalysisCell Line: A549 cells Concentration: 20 μM Incubation Time: 48 hours, 72 hours Result: Caused not only the release of cytochrome c into the cytosol, but also the activation of caspase-3 and caspase-9. In Vivo J14 (50 mg/kg; intraperitoneal injection; daily; for 16 days; BALB/c nude female mice) treatment significantly reduces the average tumor volume. The masses and weights of the primary tumors excised from the J14-treated mice are significantly lower compared with those of the control mice . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Six-week-old BALB/c nude female mice injected with A549 cells Dosage: 50 mg/kg Administration: Intraperitoneal injection; daily; for 16 days Result: Significantly reduced the growth of human lung tumor without acute toxicity. Form:Solid IC50& Target:IC50: 8.1 μM (Sulfiredoxin),ROS

J14 是一种可逆性硫氧化还原酶抑制剂，IC50 为 8.1 μM。J14 通过抑制磺胺氧化还原酶诱导氧化应激（细胞内 ROS 积累），导致细胞毒性和癌细胞死亡。

1043854-13-2

C28H25ClN4O2S

517 g/mol

45987688

C1CN(CCN1C2=CC=CC=C2Cl)C3=NC(=NC(=C3)C4=CC=CC=C4)SCC5=CC=C(C=C5)C(=O)O

4-[[4-[4-(2-chlorophenyl)piperazin-1-yl]-6-phenylpyrimidin-2-yl]sulfanylmethyl]benzoic acid

1S/C28H25ClN4O2S/c29-23-8-4-5-9-25(23)32-14-16-33(17-15-32)26-18-24(21-6-2-1-3-7-21)30-28(31-26)36-19-20-10-12-22(13-11-20)27(34)35/h1-13,18H,14-17,19H2,(H,34,35)

RSHUJZXWKLIBRE-UHFFFAOYSA-N

C1CN(CCN1C2=CC=CC=C2Cl)C3=NC(=NC(=C3)C4=CC=CC=C4)SCC5=CC=C(C=C5)C(=O)O

45987688

DMSO : ≥ 125 mg/mL (241.76 mM)

1

7

7

516.139 Da

516.139 Da

94.900 Ų

36

0

695.000

0

1

6.400