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名称

溶菌酶来源于鸡蛋白

别名

溶菌酶 | 粘肽N-乙酰胞壁质水解酶 | 胞壁质酶 | 鸡蛋清溶菌酶

英文别名

Mucopéptido N-acetilmuramoilhidrolasa | Muramidasa | Lisozima from chicken egg white

货号

L105521-100g

包装规格

100g

级别

EnzymoPure™

浓度

≥20，000U/mg

产品介绍

是一种能水解致病菌中黏多糖的碱性酶。主要通过破坏细胞壁中的N-乙酰胞壁酸和N-乙酰氨基葡糖之间的β-1,4糖苷键，使细胞壁不溶性黏多糖分解成可溶性糖肽，导致细胞壁破裂内容物逸出而使细菌溶解。溶菌酶还可与带负电荷的病毒蛋白直接结合，与DNA、RNA、脱辅基蛋白形成复盐，使病毒失活。酶分解细菌的细胞壁；用于制备原生质球。 UNIT DEFINITION ONE UNIT WILL PRODUCE A CHANGE IN A450 OF 0.001 PER MIN AT PH6.24 AT 25DEGC, USING A SUSPENSION OF MICROCOCCUS LYSODEIKTICUS AS SUBSTRATE, IN A 2.6ML REACTION MIXTURE (1CMLIGHT PATH) Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

生化机理

溶菌酶水解肽聚糖中 N-乙酰胞壁酸和 N-乙酰基-D-葡糖胺残基间的 β(1→4) 键以及壳糊精中 N-乙酰基-D-葡糖胺残基间的 β(1→4)键。革兰氏阳性细胞对该水解作用非常敏感，因为它们的细胞壁含有较高比例的肽聚糖。由于存在外膜以及比例较低的肽聚糖，革兰氏阴性细菌对该水解作用不太敏感。然而，细菌外膜中如果存在与金属离子螯合的 EDTA，那么这些细胞也可能被水解。

Safety

「No Dangerous Attributes」

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