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名称

溶壁酶来源于藤黄节杆菌

别名

细菌溶壁酶, 裂解酶

货号

L303583-1g

包装规格

级别

EnzymoPure™

浓度

≥200 units/mg

产品介绍

溶壁酶可用于重悬细胞以提取高分子量DNA用于PCR（聚合酶链式反应）指纹分析。裂解酶已用于：作为球状体形成形缓冲液成分，制备酵母核提取物消化样品制剂，用酵母进行β-葡聚糖酶促测定裂解细胞以便RNA分离产品来源：fromm Arthrobacter luteus Lyticase from Arthrobacter luteus has been used to lyse the fungal cell wall for DNA isolation. Lyticase from Arthrobacter luteus has been used: · for spheroplasting the cells · as a component of digestion solution to incubate yeast cells for digestion of the cell wall · in the enzymatic hydrolysis of the mycelium precipitate to prepare protoplasts

生化机理

溶壁酶(Lyticase)常用于真菌研究，特别是用于PCR法（聚合酶链式反应）物种鉴定。可切开糖基之间的β (1→3)和β (1→4)键。溶壁酶是一种裂解酶，用于通过诱导部分原生质球形成，从酵母细胞中提取DNA。随后再裂解原生质球以释放DNA。酵母细胞的细胞壁可能形成荚膜或耐药孢子，很难破坏，因此溶壁酶成为消化酵母细胞壁的首选方案。溶壁酶含有β-(1→;3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。它适用于多种酵母细胞，例如念珠菌(Candida)、德巴利酵母菌([5] Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。

生物活性

≥200 U/mg powder

来源

天然

Safety

「No Dangerous Attributes」

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