来自西非单叶豆（加纳籽）的凝集素

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

L755047-0.2mg

0.2mg

Isolectin B4 (BSI-B4), peroxidase conjugate, lyophilized powder

-20°C储存

超低温冰袋运输

通用蛋白质印迹方案：糖蛋白样品规格：500ng凝集素浓度： 0.1ug/ml每条泳道上样500 ng糖蛋白样品进行4-20% Bis-Tris SDS page凝胶转膜凝胶至PVDF膜上用于RIPA缓冲液（R0278 Sigma）在室温对膜进行封闭1小时在室温用RIPA缓冲液以0.1 ug/ml孵育HRP凝集素2小时用25ml RIPA缓冲液洗膜 5 x 5分钟用化学发光底物（CPS1-120）进行检测 General Western Blot Protocol: Glycoprotein sample size: 500ng Lectin Concentration: 0.1ug/ml Load samples at 500 ng of glycoprotein per lane Run 4-20% Bis-Tris SDS page gel Transfer gel to a PVDF membrane Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma) Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT Wash membrane 5 x 5 minutes with 25ml RIPA buffer Detect using chemiluminescent substrate (CPS1-120)

BS-I对末端α-D-半乳糖残基具有主要亲和力，对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。