来自 普通小麦 的凝集素（小麦）

Lectin from Triticum vulgaris (wheat)

L755095-1mg

1mg

peroxidase conjugate, lyophilized powder

-20°C储存

超低温冰袋运输

用适当的过氧化物酶底物检测含有β(1→4)-N-乙酰基-D-葡糖胺的糖蛋白通用Western Blot实验方法：糖蛋白样品规格：500ng凝集素浓度：0.1ug/ml每条泳道上样500 ng糖蛋白样品进行4-20% Bis-Tris SDS page凝胶转膜至PVDF膜上用于RIPA缓冲液（R0278 Sigma）在室温对膜进行封闭1小时在室温用RIPA缓冲液以0.1 ug/ml孵育HRP凝集素2小时用25ml RIPA缓冲液洗膜 5 x 5分钟用化学发光底物（CPS1-120）进行检测 Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate General Western Blot Protocol: Glycoprotein sample size: 500ng Lectin Concentration: 0.1ug/ml Load samples at 500 ng of glycoprotein per lane Run 4-20% Bis-Tris SDS page gel Transfer gel to a PVDF membrane Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma) Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT Wash membrane 5 x 5 minutes with 25ml RIPA buffer Detect using chemiluminescent substrate (CPS1-120)

WGA 不具有血型特异性，但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。