花生凝集素

蛋白质

花生的凝集素

PNA | peanut agglutinin

Lectin from Arachis hypogaea (peanut)

L755170-1mg

1mg

FITC conjugate

-20°C储存

超低温冰袋运输

对湿度敏感

花生凝集素已用于：测定活性精子细胞的顶体状态（存在或不存在顶体基质，对应完整或已经过顶体反应的精子）荧光染料标记PNA和B220抗体探测纵隔淋巴结(mLN)冷冻切片以检测生发中心(GC)可视化预处理视网膜切片的锥体外节应用： 凝集素是碳水化合物结合蛋白，广泛存在于真菌、植物和动物中。植物和动物凝集素的结构经过了各种不同研究。该蛋白的二级结构富含β折叠，表面具有糖类结合位点。 Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface. Application: Lectin from A rachis hypogaea (peanut) has been used: to determine the acrosomal status (presence or absence of acrosomal matrix corresponding to intact or acrosome-reacted spermatozoa) on viable sperm cells to probe cryosections of mediastinal lymph nodes (mLNs) with fluorochrome-labeled PNA and anti-B220 to detect germinal centers (GCs) to visualize the cone outer segments of pre-treated retinal sections General description Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit. Buffer：10 bicarbonate, 150 mM NaCl, pH 8.2, 0.1 mM Calcium chloride and 0.05% sodium azide

凝集素可用于糖缀合物的表征、成像和靶向研究。它用于微阵列分析，可实现高效的糖组谱分析，因其特异性作用于寡糖、糖蛋白和糖脂。植物和真菌中的凝集素可抵抗病原体/害虫(feeder)侵袭。凝集素参与宿主识别和组织粘附，从而有助于微生物致病机能。花生凝集素不会凝集正常的人类红细胞，但会强烈凝集神经氨酸酶处理的红细胞。花生凝集素具有与人血清中的抗 T 抗体相似的有效抗 T 活性。凝集素可用于区分人淋巴细胞亚群。