Lumi-Light ^PLUS 蛋白免疫印迹底物 | Lumi-Light PLUS 蛋白免疫印迹底物

L755436-100ml

100ml

pkg of 100 mL (1,000 cm2 membrane)

Lumi-Light PLUS Western 免疫印迹底物用于采用辣根过氧化物酶结合二抗对膜上印迹的抗原进行化学发光检测。并且可以非常灵敏地检测 PVDF Western 印迹膜或硝酸纤维素膜上印迹的任何抗原。Lumi-LightPLUS Western 免疫印迹底物适用于高灵敏度的 Western 免疫印迹，尤其是需要定量时。化学发光信号持续超过 9 小时，允许多次曝光。Lumi-Light PLUS 蛋白免疫印迹底物已用于 western 印迹法。应用： 随着化学发光定量技术的发展，化学发光底物也必须不断更新。例如，传统增强鲁米诺底物的光发射在 30 min 内大幅下降。相比之下，Lumi-LightPLUS 底物的化学发光信号持续超过 9 个小时，由于只需要几分钟的曝光，因此可以进行多次曝光。此外，延长暴露的能力允许检测稀有蛋白，或更高稀释度的抗原或抗体。Lumi-LightPLUS基板的发光波长为425nm。Lumi-Light 底物和 Lumi-Light PLUS 底物的比较 Lumi-Light 底物 Lumi-LightPLUS 底物灵敏度 10-50 pg 抗原 1-5 pg 抗原一抗稀释液 稀释倍数比比色法高 10 倍 与比色检测相比，稀释高达 100 倍信号稳定性 >3 小时（孵育 30 min 增强信号） >9 小时（孵育 30 min 增强信号）试验次数 ：试剂足以检测 10 个印迹，每个印迹的大小为 10 cm x 10 cm。灵敏度： 根据一抗的亲和力，在研究中可检测 1-5 pg 抗原。储存/稳定性： Lumi-LightPLUS 底物成分在 + 2 至 + 8°C 下稳定，直至标签上打印的失效日期。成分应在 + 15℃ 至 + 25℃ 下运输。 As the technology for quantifying chemiluminescence advances, the substrates for chemiluminescence must also advance. For instance, the light emission of conventional enhanced luminol substrates substantially decreases within 30 minutes. In contrast, the chemiluminescence signal of the Lumi-LightPLUS substrate persists for more than nine hours, and since exposures of only a few minutes are necessary, multiple exposures can be taken. Additionally, the ability to take extended exposures allows the detection of rare proteins, or higher dilutions of antigen or antibody to be used. The emission wavelength of the Lumi-LightPLUS substrate is 425 nm. Comparison of Lumi-Light Substrate and Lumi-Light PLUS Substrate Lumi-Light Substrate Lumi-Light PLUS Substrate Sensitivity 10-50 pg antigen 1-5 pg antigen Pimary Antibody Dilution Dilute up to 10-fold more compared to colorimetric detection Dilute up to 100-fold more compared to colorimetric detection Signal Stability >3 hours (enhance signal with a 30-minute incubation) > 9 hours (enhance signal with a 30-minute incubation) Number of tests : The reagent is sufficient for 10 blots, each with a size of 10 cm x 10 cm. Sensitivity: Depending on the affinity of the primary antibody, 1 - 5 pg of antigen can be detected in research studies. Storage/stability: The Lumi-LightPLUS substrate components are stable at +2 to +8°C until the expiration date printed on the label. Components are shipped at +15 to +25°C. Application: Lumi-Light PLUS Western Blotting Substrate has been used in western blotting. Lumi-Light PLUS Western Blotting Substrate is for the chemiluminescent detection of antigens blotted on membranes using horseradish peroxidase-conjugated secondary antibodies. It can be used for very sensitive detection of any antigen blotted on a PVDF Western Blotting Membrane or nitrocellulose membrane. Lumi-Light PLUS Western Blotting Substrate is suited for high-sensitivity western blotting, especially when quantification is required. The chemiluminescent signal persists for more than 9 hours, allowing multiple exposures to be taken.