马来酰亚胺 PEG, mPEG-MAL

Maleimide PEG, mPEG-MAL

M163928-1g

1g

MW 5000 Da

避光,-20°C储存,充氩

超低温冰袋运输

对空气敏感；对光敏感；对湿度敏感

Maleimide functionalized polyethylene glycol, Maleimide PEG is a sulfhydryl (thiol/SH) group reactive PEG derivative that can be used to selectively modify protein, peptide or any other surfaces with availablesulfhydryl groups. The reaction between maleimide and thiol proceeds readily at pH 6.5~7.5 to form stable thioether bonds. Maleimide functionalized polyethylene glycol, Maleimide PEG is a sulfhydryl (thiol/SH) group reactive PEG derivative that can be used to selectively modify protein, peptide or any other surfaces with availablesulfhydryl groups. The reaction between maleimide and thiol proceeds readily at pH 6.5~7.5 to form stable thioether bonds.

一般来说，马来酰亚胺 PEG 摩尔过量 10 到 20 倍于含硫醇材料的量，就能产生足够的共轭作用。 所需材料 Pegylation 缓冲液、pH 值为 7 的 PBS 缓冲液或 pH 值为 7 的其他不含硫醇的缓冲液。 马来酰亚胺储备液：100 mg 加入 1 mL 结合缓冲液中。 洗涤液：蒸馏水或任何水性缓冲液。 反应步骤： 将目标材料溶解在共轭缓冲液中。估计目标材料上硫醇基团的浓度。将马来酰亚胺 PEG 储存液加入到目标共轭材料中，最终浓度至少保持在 10 mg/mL。最佳共轭需要 10~50 摩尔过量的 PEG 马来酰亚胺；让混合物在室温下搅拌 2~4 小时或在 4 0C 下过夜。

Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;