Information MRT67307 is a potent and dualIKKϵandTBK1inhibitor with IC50 of 160 and 19 nM, respectively. MRT67307 potently inhibitsULK1andULK2and blocksautophagy. Targets IKKϵ (Cell-free assay); TBK1 (Cell-free assay) 160 nM; 190 nM In vitro In macrophages, MRT67307 prevents the phosphorylation of IRF3 and the production of IFNβ. In wild-type MEFs, MRT67307 enhances the IL-1-stimulated phosphorylation of p105 at Ser933 and RelA at both Ser468 and Ser536, and also enhances IL-1-stimulated activation of NF-κB-dependent gene transcription. MRT67307 increases IL-10 production and suppresses proinflammatory cytokine production via a cAMP response element-Binding protein (CREB)-regulated transcriptional coactivator (CRTC) 3 Dependent Mechanism. In addition, MRT67307 inhibit ULK and block autophagy in MEF cells. Cell Research(from reference) Cell lines：MEFs and 293T cells Concentrations：10 μM Incubation Time：1 h