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Product details
名称
支原体预防试剂 (2000X)
英文名称
Mycoplasma Prophylactic Reagent (2000X)
货号
M752155-500μl
包装规格
500μL
级别
BioReagent
浓度
2000X
储存温度
避光,-20°C储存,避免反复冻融
运输条件
超低温冰袋运输
稳定性与储存
Store at -20℃ for 24 months. Store in the dark. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
产品介绍
产品介绍: 支原体是一种大小仅为0.2-0.3μm,无细胞壁,可透过一般过滤膜(0.22-0.45μm)的原核生物,在细胞培养过程中,支原体感染发生率达到63%,因而细胞培养过程中被支原体污染是一个世界性的难题。多篇文献研究表明:当细胞(特别是传代细胞)被支原体污染后,细胞内的DNA、RNA及蛋白表达发生改变,而细胞的生长率一般并未发生显著的影响,因而细胞被支原体污染一般难以察觉。 本产品为多肽类,不像抗生素会影响细胞生长状态并使细胞产生耐药性,可解决因支原体污染而导致细胞污染,状态下降的问题,同时其对于常见的革兰氏阴性菌和阳性菌也有一定的预防作用。从而确保研究人员的研究结果真实、可信。支原体预防试剂只需1-3天就可以显著抑制支原体生长,对细胞生长几乎无影响,完美兼具高效与温和预防细胞污染物的特点,最大程度上挽救您的细胞。 储存条件 -20℃避光保存,保质期24个月。 使用说明 请在收到货后,将试剂管瞬时离心(3000rpm,3~5s)保存。 1、使用支原体预防剂处理时,建议现配现用,建议保持细胞密度在50%~60%。推荐支原体预防剂的稀释比例为1:2000,例如:20mL的培养基加入10μL的支原体预防剂。 2、建议根据细胞的周期不一样,每2-4天处理一次,或长期处理,以预防支原体污染。 注意事项 1、使用本试剂前请仔细阅读说明书。 2、整个实验,应规范操作,包括反应体系的配制、样本处理及加样。 3、本产品仅用于科研用途,不可用于诊断、治疗、临床家庭及其他用途。
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Research chemical 500μL

Research chemical 500μL; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 500μL.

item number:M752155-500μl
Product model:500μL
level: 500μL
Lead Time:30days
sold 708 Items
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Specifications:

500μL

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