Mucinase StcE

酶

粘蛋白特异性蛋白酶StcE

Mucin-specific protease StcE, secreted protease of C1 esterase inhibitor

Mucinase StcE

M755176-100μg

100μg

重组

mass spec suitable, from EHEC, powder

-20°C储存

超低温冰袋运输

该产品在零下 20 摄氏度冻干保存时，其活性可保持至少 2 年

For use as a tool in biochemical research and biomarker discovery, particularly focused on glycobiology, glycoproteomics, glyco-oncology. Shown to be useful in LC-MS/MS workflows应用： Mucinase StcE is a metalloprotease that can be used to digest densely O-glycosylated mucins. The consensus sequence for cleavage is S/T*-X-S/T, where the cleavage happens on the amino side of the 2nd Serine/Threonine, and X can be any amino acid, or none though with lower efficiency. The P2 position (*) is found to be always occupied in cleavage products, and occupancy of both P1 and P1’ positions do not interfere with cleavage. While Mucinase StcE is active against Mucins and other mucin-domain containing glycoproteins such as CD43 or PSGL-1, it is not active against O-glycoproteins outside of the Mucin family, such as fetuin or GspB736flag1. Mucinase StcE is a metalloprotease that can be used to digest densely O-glycosylated mucins. The consensus sequence for cleavage is S/T*-X-S/T, where the cleavage happens on the amino side of the 2 nd Serine/Threonine, and X can be any amino acid, or none though with lower efficiency. The P2 position (*) is found to be always occupied in cleavage products, and occupancy of both P1 and P1’ positions do not interfere with cleavage. While Mucinase StcE is active against Mucins and other mucin-domain containing glycoproteins such as CD43 or PSGL-1, it is not active against O-glycoproteins outside of the Mucin family, such as fetuin or GspB736flag 1 . Application: For use as a tool in biochemical research and biomarker discovery, particularly focused on glycobiology, glycoproteomics, glyco-oncology. Shown to be useful in LC-MS/MS workflows

粘蛋白是一种大量 O-糖基化的蛋白质，存在于许多组织中，如粘膜和肠衬。粘蛋白的功能之一是作为上皮组织的保护层。 已知致病菌肠出血性大肠杆菌（EHEC）会通过酶的方式攻击这些组织。 其中一种被发现参与其中的酶是一种名为 C1 酯酶抑制剂分泌蛋白酶（StcE）（又名粘蛋白酶 StcE）的蛋白酶。 这种酶能特异性识别糖基化密集的粘蛋白和粘蛋白域，并以蛋白水解方式裂解肽骨，从而增强病原微生物的毒力。