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名称

网状纤维染色液 (改良Gordon-Sweets法)

英文名称

Reticular Fibre Staining Solution (Gordon-Sweets)

货号

M774851-6×50ml

包装规格

6×50ml

级别

BioReagent

储存温度

2-8°C储存,避光

运输条件

冰袋运输

稳定性与储存

Store at 2-8℃ long term (6 months). Store in the dark.

敏感性

Light-sensitive

产品介绍

网状纤维 (Reticular fiber) 是网状结缔组织内的一种纤维，由网状细胞所产生，直径多在0.2-1.0μm，有韧性而没有弹性。网状纤维的染色方法很多，但染色原理基本一致，大都采用氨银浸法。改良 Gordon-Sweets 染色原理是利用氨银液易被组织吸附与组织的蛋白质结合，经甲醛还原成黑色或棕黑色的金属银，沉积于组织内及其表面。传统方法中还原后先采用氯化金调色，再用硫代硫酸钠溶液洗去组织上未还原的银盐，本改良法省略该步骤，使网状纤维对比得更清晰。网状纤维染色液 (改良Gordon-Sweets法) 主要经过氧化、漂白、媒染、浸银、还原、复染等步骤，与改良Gomori法不同之处在于前者采用酸性氧化剂和核固红复染液。冰冻切片、低温切片和火棉胶切片均可用于网状纤维染色。各种固定液均可采用，重金属汞盐或锇盐固定液偶尔会产生一些非特异性银背景。常用于鉴别肿瘤的性质和来源、癌与肉瘤、淋巴肉瘤与网状细胞肉瘤、血管内皮瘤与血管外皮瘤、骨尤文瘤与骨网状细胞肉瘤、脑膜瘤与星形细胞瘤、恶性神经鞘瘤及早期浸润癌等。 M774851

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