核酸酶S1 来源于米曲霉

Endonuclease S1

Endonuclease S1

N128634-10KU

10KU

EnzymoPure™

≥100,000-500,000 units/ml

Product Description Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol. Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS. Product Application Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.

从黑曲霉（Aspergillus oryzae）中分离出的核酸酶 S1 对单链 DNA 和 RNA 的磷酸二酯键具有内切和外切水解活性，可产生 5'-phosphomononucleotide 和 5'-phosphooligonucleotide 终产物。它可用于消化 RNA 和 DNA 双链中未退火的多核苷酸尾部和发夹环，也可用于将超螺旋 DNA 转换为线性形式。