中性蛋白酶 NB 来源于溶组织梭菌

N278984-100U

100U

EnzymoPure™

≥ 0.50 U/mg

Description Neutral protease from Clostridium histolyticum is a proteolytic enzyme and therefore can be used for isolation of various cell types by tissue dissociation. Applications Neutral protease suitable for cell isolation from various tissue types for clinical applications. Specifications Contains chromatographically purified neutral protease. Vial contains not less than 100 DMC U of neutral protease. Neutral protease activity (DMC): ≥ 0.50 U/mg Endotoxin: ≤ 100 EU**/mg (Ph. Eur.) Largely free of collagenolytic activity. Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.). Manufactured according to EU GMP guidelines. Mr ca. 34 000 • CAS [9001-92-7] References and Definitions Unit definition: *Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1). **Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)). References 1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93. Description Neutral protease from Clostridium histolyticum is a proteolytic enzyme and therefore can be used for isolation of various cell types by tissue dissociation. Applications Neutral protease suitable for cell isolation from various tissue types for clinical applications. Specifications Contains chromatographically purified neutral protease. Vial contains not less than 100 DMC U of neutral protease. Neutral protease activity (DMC): ≥ 0.50 U/mg Endotoxin: ≤ 100 EU**/mg (Ph. Eur.) Largely free of collagenolytic activity. Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.). Manufactured according to EU GMP guidelines. Mr ca. 34 000 • CAS [9001-92-7] References and Definitions Unit definition: *Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1). **Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)). References 1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.