NerveGreenTM C4神经元荧光探针

Neurogreentm C4 neuron fluorescent probe

N598339-5mg

5mg

2-8°C储存,避光

冰袋运输

神经末梢探针是一系列阳离子型苯乙烯基荧光染料，用于跟踪神经肌肉接头或突触的突触活动。这类染料通常具有亲脂性尾部（两个碳链）和带阳离子的高亲水性头部。NerveGreenTM系列探针是具有单个双键的染料。 阳离子苯乙烯基染料是通过活性依赖性染色突触小泡来发挥功能。染料与细胞或组织共孵育时，染料的水相部分没有荧光，而染料的亲脂性尾部插入细胞膜并呈现强荧光。神经刺激后，在进行胞吞作用时，染料被包裹在囊泡内，因此，洗去细胞表面附着的染料后，荧光信号强弱表示新形成的囊泡的数量的多少。反之，在胞吐作用时，染料与神经递质一起从囊泡释放，导致荧光信号减少。因此，荧光强度的变化反映了胞吞/胞吐或突触活动的情况。内吞过程中荧光增加的速率——“结合速率”和胞吐过程中荧光减少的速率 ——“解离速率”因染料种类而异。通常，具有较长亲脂性尾部和更多双键的染料对膜具有较高的亲和力，因此具有较高的结合速率和较低的解离速率。 本产品等同于 FM1-43，NerveGreenTM C4 是一种广泛使用的绿色荧光染料，通过染色突触的突触囊泡或神经肌肉接头来跟踪突触活动。结合使用红色荧光染料 NerveRedTM，突触和神经肌肉接头可以独立两个颜色成像。 注意事项： 1. 荧光染料应尽量注意避光，以减缓荧光淬灭。 2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。 应用范围： 神经荧光染料 Product introduction： Nerve terminal probes are a series of cationic styryl fluorescent dyes used to track synaptic activity at neuromuscular junctions or synapses. Such dyes usually have lipophilic tails (two carbon chains) and highly hydrophilic heads with cations. The nervegreentm series of probes are dyes with a single double bond. Cationic styryl dyes function by activity dependent staining of synaptic vesicles. When the dye is co incubated with cells or tissues, the aqueous part of the dye has no fluorescence, while the lipophilic tail of the dye inserts into the cell membrane and exhibits strong fluorescence. After nerve stimulation, the dye is encapsulated in the vesicles during endocytosis. Therefore, after washing the dye attached to the cell surface, the fluorescence signal intensity indicates the number of newly formed vesicles. On the contrary, during exocytosis, the dye is released from the vesicles together with neurotransmitters, resulting in the reduction of fluorescence signal. Therefore, the change in fluorescence intensity reflects the situation of endocytosis / exocytosis or synaptic activity. The rate of fluorescence increase during endocytosis - "binding rate" and the rate of fluorescence decrease during exocytosis - "dissociation rate" vary according to the dye type. In general, dyes with longer lipophilic tails and more double bonds have higher affinity for membranes and thus higher binding rates and lower dissociation rates. This product is equivalent to FM1-43. Nervegreentm C4 is a widely used green fluorescent dye that tracks synaptic activity by staining synaptic vesicles or neuromuscular junctions at synapses. Using the red fluorescent dye nerveredtm in combination, synapses and neuromuscular junctions can be imaged in two colors independently. Matters needing attention： 1. fluorescent dyes should be protected from light as much as possible to slow down fluorescence quenching. 2. for your safety and health, please wear experimental clothes and disposable gloves. Scope of application： Neurofluorescent dye