NHS-Phosphine

国家卫生系统-磷化氢

NHS-Phosphine

N638958-10mg

10mg

避光,-20°C储存,干燥

超低温冰袋运输

Product introduction： NHS-Phosphine is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules. Features of NHS-Phosphine: • Soluble —reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples • Compatible —reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents • Specific —NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides) • Chemoselective —azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine group (PPh2) reacts with azide-labeled molecules by a mechanism known asStaudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, this NHS-Phosphine reagent facilitates a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways. • Reactive Moiety: NHS Ester,Phosphine • Crosslinker Type: Heterobifunctional • Form: Powder: • Spacer: Short( Labeling Method: Chemical Labeling • Spacer Arm Length: 5.4 Å

461.4 g/mol

DMF,DMSO

Amine-Azide