应用： 微生物学检验试剂，用于细胞色素氧化酶阳性好氧微生物的分类；对于氧化酶试剂，使用 1% 的 N,N,N′,N′-四甲基对苯二胺二盐酸盐的水溶液 N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride(TMPD) is an oxidizable compound that serves as a reducing co-substrate for heme peroxidases. TMPD undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm. Thus, the stoichiometry of oxidation is 2 moles of TMPD oxidized per mole of hydroperoxide reduced by the peroxidase. The extinction coefficient of the oxidized TMPD at 611 nm is 12,200.2 TMPD is also used for the detection of peroxidases on polyacrylamide gels. An oxidizable compound Test reagent in microbiology for the classification of cytochrome oxidase positive aerobic microorganisms.; For oxidase reagent, use a 1% aqueous solution of N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride