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名称

尼氏染色液 (亚甲蓝法)

分子类型

生物试剂/缓冲液

英文名称

Nissl staining solution (methylene blue)

货号

N774532-3×50ml

包装规格

3×50ml

级别

BioReagent

储存温度

避光,室温

运输条件

冰袋运输

稳定性与储存

Store at room temperature long term (6 months). Store in the dark.

敏感性

Light-sensitive

产品介绍

神经元细胞体包括一个具有皱褶核膜的大细胞核、稀疏的染色质和一个明显的核仁。在细胞体中细胞质是尼氏颗粒，即能够代表粗面内质网并在很多神经元中产生特异的斑点状嗜碱性表现的嗜碱性颗粒。尼氏颗粒可以用很多染色来显示如中性红、亚甲基蓝、甲苯胺蓝和甲基紫等。染色的变异、pH和分化的时间使一些染色既可以仅突出尼氏物质，也可以显示神经元的细胞核和神经胶质。尼氏体（Nissl body）或称尼氏小体是分布于神经细胞胞质内的三角形或椭圆形小块状物质，能被碱性染料如硫堇、亚甲蓝、甲苯胺蓝和焦油紫等染料染成紫蓝色。各种神经细胞内都含有尼氏体，但其形状、数量、分布位置常常不同。尼氏体也存在于树突中，但不在于轴突和包体的轴丘。尼氏体会因为生理状态的变化而变化，尼氏体是神经元内合成蛋白质合成的重要部位，当神经元受到刺激后，包体内的尼氏体会明显减少。尼氏染色液（亚甲蓝法）主要优点是操作简便、染色稳定、适用范围广，可以用于石蜡组织切片的尼氏物质、神经元等的染色，尼氏体的存在和消失是神经细胞是否受损的重要指标，当发生脑炎、脑缺血、轴突反应等情况时，尼氏体会发生溶解甚至消失。 N774532

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