JAK2抑制剂V，Z3是可渗透细胞的吡啶基丁酮化合物，可同时抑制JAK2和活性V167F突变细胞自磷酸化。在无细胞激酶测定中，JAK2抑制剂V，Z3对COS细胞或SRC中的细胞Tyk 2自磷酸化没有作用。JAK2抑制剂V，Z3通过阻止G1-S过渡来抑制红白血病细胞系HEL的JAK2-V167F依赖性增殖，并显示可有效减少细胞因子刺激的Jak2-V617F阳性和Jak2-F537I阳性的生长骨髓增生异常患者的原代造血祖细胞。 JAK2 Inhibitor V, Z3 is a cell-permeable pyridinylbutanone compound that inhibits both JAK2 and the active V167F mutant cellular autophosphorylation. JAK2 Inhibitor V, Z3 displays no effect against cellular Tyk 2 autophosphorylation in COS cells or SRC in cell-free kinase assays. JAK2 Inhibitor V, Z3 suppresses the JAK2-V167F-dependent proliferation of the erythroleukemia cell line HEL by blocking the G1-S transition and is shown to effectively reduce the cytokine-stimulated growth of both Jak2-V617F-positive and Jak2-F537I-positive primary hematopoietic progenitor cells from myeloproliferative disorder patients.