ODN 2007, a class B CpG ODN (oligodeoxynucleotide), is a Toll-like receptor ( TLR ) ligand. ODN 2007 can be used as an immunomodulator, vaccine adjuvant, and enhance immune responses in mammals, fish, and humans. ODN 2007 sequence: 5'-TCGTCGTTGTCGTTTTGTCGTT-3' . In Vitro ODN 2007 (1 or 5 μg/mL, 3-18 h) induces significantly chicken macrophage interferon IFN-γ and IFN-β expression. ODN 2007 (10 μg/mL, 0.5-12 h) can increase levels of phosphorylation of ERK2 and AKT, stimulate the production of NO. MCE has not independently confirmed the accuracy of these methods. They are for reference only. RT-PCRCell Line: MQ-NCSU cells (a chicken macrophage cell line) Concentration: 1 μg/mL, 5 μg/mL Incubation Time: 3 h, 12 h, 18 h Result: Significantly stimulated an increase in transcriptional expression of IFN-γ after 3 hours regardless of the dose. Increased the mRNA expression of IL-1β at 5 μg/mL high dose regardless of the time point. Western Blot AnalysisCell Line: HD11, a replication-deficient avian leukemia virus MC29-transformed macrophage-like cell line Concentration: 10 μg/mL Incubation Time: 0.5 h, 1 h, 3 h, 6 h, 12 h Result: Resulted in a significant increase in ERK2 and AKT phosphorylation levels and increased IFN-γ, IL-6 and MIP-3α mRNA levels. Stimulated an increase in the level of NO. In Vivo ODN 2007 (intraperitoneal injection, 1 μg, once) can enhance the immune response by regulating the expression of immune system-related genes in zebrafish infected with Vibrio traumaticus FJ03-X2. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Zebrafish infected with Vibrio traumaticus FJ03-X2Dosage: 1 μg Administration: Intraperitoneal injection; once Result: Showed a cumulative mortality rate of 15.0% in treated group while the control group reached 42.1%. Showed a significant decrease in the expression levels of all intestinal immune-related genes such as TNF、IFNg1-2、IL-1β、IL-10, especially IL-1β decreased by 99.88%. Form:Solid