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名称

油红O染色液试剂盒 (培养细胞专用)

分子类型

生物试剂/缓冲液

别名

油红O染色液 (培养细胞专用) | 油红O染色液 (细胞专用) | 脂肪染色

英文别名

Oil Red O Staining Solution

英文名称

Oil Red O Staining Kit (For Cell Culture)

货号

O774830-4×20ml

包装规格

4×20ml

级别

BioReagent

储存温度

2-8°C储存,避光,室温

运输条件

冰袋运输

稳定性与储存

Store at 2-8℃ long term (12 months). Store in the dark.

敏感性

Light-sensitive

产品介绍

脂质 (Lipid) 是中性脂肪、类脂及其衍生物的总称，其共同的物理特性是不溶于水，易溶于有机溶剂 (如乙醇、乙醚等)。人体的脂肪主要有两种：1、储存脂肪，如中性脂肪，主要分布于皮下、肾、胰腺等部位。2、结构脂肪，如类脂 (磷脂、糖脂、胆固醇等)，主要分布于细胞内。中性脂肪 (Neutralfat) 是由三分子脂肪酸和一分子甘油组成的脂类，呈中性。中性脂肪染色经常采用苏丹Ⅱ、苏丹Ⅲ、苏丹Ⅳ、苏丹黑B、油红O法等。传统方法采用苏丹染料，最近发现偶氮染料油红O更适合脂肪的染色。油红O是很强的脂溶剂和染脂剂，较易与甘油三脂结合呈小脂滴状，与磷脂结合力稍差，其染色原理一般认为是物理上的溶液作用或吸附作用，借溶液作用使脂肪染色。油红O染色液 (培养细胞专用) 主要用于显示人工培养细胞的脂肪变性和类脂质的异常沉着，细胞内出现多数中性脂肪滴，鉴别培养细胞中所发生的变化及其性质。标本不采用含有乙醇的固定液，脂肪的阳性染色结果呈橘黄至红色，但具体颜色因脂质浓度而定。 O774830

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