磷酸酶(酸性)来源于小麦胚芽

酸性磷酸酶

3-(2-propynyloxy)propan-2-ol | SCHEMBL4278701 | 1-(PROP-2-YN-1-YLOXY)PROPAN-2-OL | DTXSID701009098 | 1-prop-2-ynoxypropan-2-ol | 1-Prop-2-inyl-naphthalin | Acidphosphatase | 1-(prop-2-ynyloxy)propan-2-ol | Phosphatase, acid | AKOS015917690 | 9001-77-8

P128633-1g

1g

EnzymoPure™

≥0.15 units/mg dry weight

Acid phosphatase is an esterase with broad activity at an optimal pH below 7.0. There are three isozymes, EI, EII, and EIII of similar molecular weight (55 kDa± 5 kDa). Their optimum pH's are 5.5, 4.5, and 4.0 respectively. Acid phosphatase activity was observed by Teller Aladdin Library Archives in 1954 in preparations of a wheat germ lipase described by Singer JBC, 174, 11, in 1948. Equivalent commercial preparations have been distributed labeled as lipase and acid phosphatase thus generating some confusion. Subsequent work has confirmed that the non-specific esterase activity of the wheat germ preparation may be measured both as lipase (triacetin as substrate) and phosphatase. The enzyme assay is based on the work of Brandenberger and Hanson (Helv. Chim. Acta, 36, 900, 1953) and Hofstee ( Arch. Biochem. Biophys., 51, 239, 1954).Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Acid phosphatase is an esterase with broad activity at an optimal pH below 7.0. There are three isozymes, EI, EII, and EIII of similar molecular weight (55 kDa± 5 kDa). Their optimum pH's are 5.5, 4.5, and 4.0 respectively. Acid phosphatase activity was observed by Teller Aladdin Library Archives in 1954 in preparations of a wheat germ lipase described by Singer JBC, 174, 11, in 1948. Equivalent commercial preparations have been distributed labeled as lipase and acid phosphatase thus generating some confusion. Subsequent work has confirmed that the non-specific esterase activity of the wheat germ preparation may be measured both as lipase (triacetin as substrate) and phosphatase. The enzyme assay is based on the work of Brandenberger and Hanson (Helv. Chim. Acta, 36, 900, 1953) and Hofstee ( Arch. Biochem. Biophys., 51, 239, 1954). Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Characteristics of Acid Phosphatase from Wheat Germ: Molecular weight: 55,000 ± 5,000 (Verjee 1969). Composition: Three isozymes of closely similar molecular weights have been reported by Verjee (1969): EI, EII, and EIII. See also Brouillard and Ouellet (1965). Optimal pH: EI - 5.5, EII - 4.5, and EIII - 4.0. (Verjee 1969). Specificity: The enzyme has a broad esterase activity. See Joyce and Grisolia (1960). It shows highest activity for pyrophosphate. Inhibitors: Fluoride, molybdate and orthophosphate (Verjee 1969).

植物酸性磷酸酶（APase）定位于细胞质、液泡和细胞壁。植物酸性磷酸酶对于获取磷酸盐以调动土壤中的有机磷酸盐非常重要。磷酸盐饥饿会诱导酸性磷酸酶的生成。酸性磷酸酶来源于发芽过程中的胚乳和颖果组织。它能水解植酸、ATP、蛋白质磷酸盐和核苷酸磷酸盐。它在一般代谢反应中发挥作用。小麦胚 AP 酶由四种同工酶组成。小麦胚芽 AP 酶的分子质量为 58 kDa（凝胶过滤），最适 pH 值为 5.7，pH 值范围为 4.0-7.0，最适温度为 45 °C。