预染蛋白双色Marker（10-250kDa）

P598077-25μl

25μl

​ 预染蛋白双色Marker（10-250 kDa）由11个蛋白条带组成，包括70 kDa和25 kDa两条结合红色染料的参考带；可运用于监测电泳过程中蛋白分离情况和Western Blot实验中的转膜效率以及判断目标蛋白的分子量。不同电泳缓冲条件下，各条带指示分子量(kDa) 使用方法： 1. 加样前在室温放置数分钟，解冻后，轻轻摇匀，以确保溶液混合均匀； 2. 取用适量体积加样于凝胶孔，推荐用量如下： 0.75~1.0 mm 凝胶，上样 5 μL/孔； 1.5 mm 凝胶，上样 10μL/孔。也可根据情况适当减少加样量。 注意事项： 1. 本品可以直接使用，无需加热煮沸； 2. 可将本产品分装后，-20℃保存，避免反复冻融及污染；3. 在不同凝胶和缓冲液系统中，预染蛋白质的迁移率会有所差别，可用非预染蛋白质分子量标准进行校正，并按校正后的结果正常使用 应用范围： 蛋白Maker ​ The pre stained protein two-color marker (10-250 kDa) consists of 11 protein bands, including two reference bands of 70 kDa and 25 kDa that bind red dye; It can be used to monitor the protein separation in the process of electrophoresis and the membrane transfer efficiency in Western blot experiments, as well as to judge the molecular weight of the target protein. Under different electrophoresis buffer conditions, each band indicated molecular weight ( kDa ). Instruction： 1.Place it at room temperature for several minutes before adding the sample, shake it gently after thawing to ensure that the solution is mixed evenly ; 2.Appropriate volume was added to the gel pores, and the recommended dosage was as follows : 0.75 ~ 1.0 mm gel, 5 μL / well ; 1.5 mm gel, 10 μL / well. The amount of sample can also be appropriately reduced according to the situation. Matters needing attention： 1. this product can be used directly without heating and boiling; 2. the product can be stored at -20 ℃ after subpackaging to avoid repeated freeze-thaw and pollution; 3. in different gel and buffer systems, the mobility of pre stained proteins will be different, which can be corrected according to the molecular weight standard of non pre stained proteins and used normally according to the corrected results Scope of application： Protein maker