蛋白酶 来源于灰色链霉菌

链霉蛋白酶E

P736615-250mg

250mg

EnzymoPure™

~6000 units/g protein

可用于不溶性蛋白质的蛋白水解和蛋白结构研究。 可用于研究蛋白酶作为链霉素生产中副产物的产生。 选择水解谷氨酸或天冬氨酸的羧基侧的肽键。 可用于降解柞蚕丝素蛋白膜。 可用于核酸的分离过程 Pronase is a mixture of several nonspecific endo- and exoproteases that digest proteins down to single amino acids. Application: Use Pronase to completely hydrolyze proteins in research applications. Pronase is used for the degradation of proteins during the isolation of DNA and RNA, such as in the extraction of phage DNA or the isolation of plasmid DNA. It is not necessary to let pronase self-digest prior to use. It is also used in histochemistry and cell culture for tissue dissociation in conjunction with collagenase and trypsin，and for the production of glycopeptides from purified glycoproteins. It has been used for the dechorionation of Zebrafish embryos.

Pronase 具有广泛的特异性。因此，它常用于需要广泛或完全降解蛋白质的情况。其应用包括细胞器中的蛋白质分析、在 DNA 和 RNA 分离过程中去除蛋白质，以及生产用于氨基酸分析的蛋白质水解物。此外，Pronase 在分散成纤维细胞系方面的能力超过了胰蛋白酶，在单层分离和创建单细胞悬浮液方面都表现出色。