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Product details
名称
UltraBio™免疫染色一抗稀释液
英文名称
UltraBio™ Primary Antibody Dilution Buffer for Immunol Staining
货号
P751641-100ml
包装规格
100ml
储存温度
-20°C储存
运输条件
超低温冰袋运输
稳定性与储存
4℃保存,一年有效。长期不使用可以-20℃保存。
产品介绍
阿拉丁生产的UltraBio™免疫染色一抗稀释液(UltraBio™ Primary Antibody Dilution Buffer for Immunol Staining)是最新一代的高效一抗稀释液,总体效果显著优于传统的基于BSA(牛血清白蛋白)、血清等的一抗稀释液,可以用于免疫荧光(Immunofluorescence, IF)、免疫组化(Immunohistochemistry, IHC)和免疫细胞化学(Immunocytochemistry, IC)等免疫染色时一抗的稀释和配制。UltraBio™免疫染色一抗稀释液可以有效节约一抗。本产品稀释和配制的一抗可以在4℃保存和使用不少于1个月,并且可以反复多次用于免疫染色,利于节约一抗。UltraBio™免疫染色一抗稀释液稀释的一抗孵育后背景极低。本封闭液不含血清和白蛋白,确保极高的信噪比。UltraBio™免疫染色一抗稀释液兼容性好。后续兼容辣根过氧化物酶(Horseradish peroxidase,HRP)、碱性磷酸酶和生物素标记的二抗。本产品不含生物素,不会干扰基于生物素的检测。UltraBio™免疫染色一抗稀释液使用便捷。本产品无需添加任何额外的试剂,可以直接用于一抗的稀释。 图1.UltraBio™免疫染色一抗稀释液用于免疫荧光(IF)和免疫细胞化学(IC)的检测效果图。上图为HeLa细胞用一抗α-Tubulin Rabbit Polyclonal Antibody ,和二抗Cy3标记的山羊抗兔IgG(H+L) 进行荧光染色(左图)或二抗辣根过氧化物酶标记山羊抗兔IgG(H+L) 并用DAB显色(右图)的检测效果图。左图中可见非常清晰的红色荧光的微管形态,右图中可见非常清晰的棕色微管形态。实际实验结果会因样品、抗体、实验条件等的不同而存在差异,图中数据仅供参考。按照每次一抗稀释需要5-10ml一抗稀释液计算,一个包装的UltraBio™免疫染色一抗稀释液可以稀释10-20次一抗。 注意事项 : 为进一步提高信噪比,推荐同时使用QuickBlock?免疫染色封闭液、QuickBlock?免疫荧光染色二抗稀释液和QuickBlock?免疫组化染色二抗稀释液用于封闭及二抗的稀释。为了能使稀释的一抗可以反复多次使用,一抗孵育结束后稀释的一抗应立即存放在4℃,以便于后续重复使用。本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。为了您的安全和健康,请穿实验服并戴一次性手套操作。 使用说明 : 参考所用一抗的说明,以及样品中目的蛋白的含量,按照适当比例例如1:1000、1:500等稀释一抗。一抗稀释后即可直接用于免疫染色。一次免疫染色结束后,可以回收稀释的一抗,4℃保存,以用于下次的免疫染色。详细的免疫染色操作可以参考阿拉丁相关网页:http://www.aladdin-e.com/immunol-staining.htm。 To further improve the signal-to-noise ratio, we recommend using QuickBlock™ Blocking Buffer for Immunol Staining , QuickBlock™ Secondary Antibody Dilution Buffer for Immunofluorescence and QuickBlock™ Secondary Antibody Dilution Buffer for Immunohistochemistry in conjunction to block samples and dilute secondary antibodies.The diluted primary antibody should be stored at 4℃ immediately after use to enable multiple reuses.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation. Instructions for Use : Dilute the primary antibody with this product at an appropriate ratio according to manufacture's instructions of the primary antibody as well as the content of target protein in samples. The diluted primary antibody can be used directly for immunostaining. The diluted primary antibody can be recovered after use and stored at 4℃ for later use.
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Research chemical 100ml

Research chemical 100ml; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: 100ml.

item number:P751641-100ml
Product model:100ml
level: 100ml
Lead Time:30days
sold 105 Items
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