蛋白酶磷酸酶抑制剂混合物 （100×, 不含EDTA）

P752090-1ml

1ml

BioReagent

100×

本产品由优化后的多种蛋白酶抑制剂和磷酸酶抑制剂混合而成，在抽提和纯化蛋白时，仅使用一种溶液即可有效防止蛋白被内源性蛋白酶和磷酸酶降解。其成分含有Aprotinin、Bestatin、Leupetin、E-64、NaF、Sodium Orthovanadate、Sodium Pyrophosphate以及β-glycerophosphate等多种蛋白酶和磷酸酶抑制剂，可广泛应用于蛋白纯化、蛋白免疫印迹、免疫共沉淀、免疫荧光、免疫组织化学以及激酶测定等研究。因不含会导致峰值偏移的AEBSF等物质，本产品可与质谱分析(MS)兼容。 使用说明 1、本产品可用于各种蛋白质含量或其活性分析实验(Western Blot、Co-IP、Pull-down、IF和IHC等)，保护蛋白质免受蛋白酶和磷酸酶的降解； 2、将本品以1:100（体积比）的稀释比加入到目标溶液中（如细胞裂解液，蛋白提取液等）即可。 注意事项 1、不同来源的样品中蛋白酶和磷酸酶的含量各不相同，1:100为推荐的常规剂量。实际使用中，如发现蛋白酶或磷酸酶含量较高，可适当提高本产品的使用浓度； 2、本产品不含EDTA，如需使用，需另行添加； 3、本产品不含Pepstatin A，如需抑制酸性蛋白酶，需另行添加Pepstatin A； 4、本产品为高浓度溶液，长期存放可能会出现微量晶体状沉淀，此为正常现象，不影响实验结果； 5、为了您的安全和健康，请穿实验服并戴一次性手套操作。所有抑制剂对人体均有毒，请勿直接与皮肤接触。如不小心接触皮肤，请立即用大量清水冲洗。如不慎吞食，应立即就医处理； 6、本产品仅限科研使用。 This product is an optimized blend of multiple protease and phosphatase inhibitors. During protein extraction and purification, a single solution effectively prevents protein degradation by endogenous proteases and phosphatases. Its components include Aprotinin, Bestatin, Leupetin, E-64, NaF, Sodium Orthovanadate, Sodium Pyrophosphate, and β-glycerophosphate, among other protease and phosphatase inhibitors. It is widely applicable for protein purification, Western blotting, co-immunoprecipitation (Co-IP), immunofluorescence (IF), immunohistochemistry (IHC), and kinase assays. As it does not contain interference-prone substances like AEBSF, this product is compatible with mass spectrometry (MS) analysis. Protocol Suitable for various protein quantification or activity analysis experiments (e.g., Western Blot, Co-IP, Pull-down, IF, IHC) to protect proteins from degradation by proteases and phosphatases. Dilute the inhibitor cocktail 1:100 (v/v) into the target solution (e.g., cell lysis buffer, protein extraction buffer). Precautions The recommended dilution ratio of 1:100 is suitable for general use. Adjust the concentration accordingly if the sample contains high levels of proteases or phosphatases. This product does not contain EDTA. If required, add EDTA separately. This product does not contain Pepstatin A. To inhibit acid proteases, add Pepstatin A separately. This is a high-concentration solution. Long-term storage may result in the formation of trace crystalline precipitates, which is normal and does not affect experimental results. For safety, wear a lab coat and disposable gloves during handling. All inhibitors are toxic to humans—avoid direct skin contact. In case of contact, rinse immediately with plenty of water. If ingested, seek medical attention immediately. For research use only.