木瓜蛋白酶 来源于番木瓜

P754923-50mg

50mg

solution, light brown,≥10 U/mg protein (~25 mg/ml)

从番木瓜提取的木瓜蛋白酶已用于：酶解大鼠皮质以提取用于细胞培养的星形胶质细胞研究对羊驼精浆粘度和精子功能的影响研究对犀牛精子产量和低温存活率的影响木瓜蛋白酶被用作嫩肉精。它还被用于在原代细胞培养物制备中分解组织。应用： 木瓜蛋白酶是一种天然存在于木瓜（Carica papaya L.）中的蛋白水解酶。可以从切开的未成熟木瓜的乳胶中获得。木瓜蛋白酶属于半胱氨酸蛋白酶家族。它是一种球状单链蛋白质，由四个二硫桥和催化基础残基组成。木瓜蛋白酶由全α-结构域和反平行片β-结构域组成。 Papain is a proteolytic enzyme and is naturally occurring in papaya ( Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain. Application: Papain from Carica papaya has been used: in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture to study its effects on Alpaca seminal plasma viscosity and sperm function to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.

木瓜蛋白酶对许多生物体的生物过程起重要作用。它对蛋白质、氨基酸酯和酰胺连接以及短链肽具有蛋白水解活性。木瓜蛋白酶倾向于切割碱性氨基酸如精氨酸、苯丙氨酸和赖氨酸的肽键。它在药物发现、制药、食品 、烘焙和乳制品行业有广泛的应用。木瓜蛋白酶是一种可切割肽键的半胱氨酸蛋白酶，并且已知可切割抗体的Fc部分。