Share to
Details
Safety
Related
Technical Documents
Articles
reviews
Consult
returns
Product details
名称
重组糖基肽酶F
别名
N-糖苷酶F | 糖苷酶F | 肽N-糖苷酶
英文别名
N-Glycosidase F | Peptide N-glycosidase | peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase | glycopeptidase | glycopeptide N-glycosidase | N-glycanase | N-oligosaccharide glycopeptidase | ngl
货号
P755115-15KU
包装规格
15KU
级别
生物活性
浓度
≥95%(SDS-PAGE&SEC-HPLC)
产品介绍
PNGase F全称肽 N-糖苷酶F,基因来源于Elizabethkingia miricola。PNGase F 是一种胺酶,切割糖蛋白上的 N-连接糖链。切割的糖型有:高甘露糖、杂合和复杂寡糖;切割位点为:最内侧的 N-乙酰葡糖胺和天冬酰胺之间的糖苷键,能够将绝大多数 N-糖链(除含 α-1,3 连接的核心岩藻糖)。适用于蛋白质组学及糖生物学研究中糖蛋白上 N-连寡糖链的有效释放。 反应式 最佳反应条件: 最适反应 pH:7.5;热失活条件:75℃处理10 min。 组分和说明 P755115
生化机理
从糖蛋白切割整个聚糖,前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。
生物活性
≥30000U/mg protein (150000U/mL) ,单位定义:10 μl 的反应体系中,37℃反应 1 小时从10 μg 变性RNase B中除去超过95%的碳水化合物所需要的酶量。
来源
重组表达
蛋白标签
N-His
SDS-PAGE
35 kDa
表达系统
E. coli Accession #: P21163 | E. coli
种属
伊丽莎白病(Elizabethkingia miricola)
Safety
「No Dangerous Attributes」
Related
「No upstream and downstream information yet」
Technical Documents
「No technical documents」
Articles
「No related articles yet」
user reviews
Loading...
Consultation

Warm reminder:

Because the manufacturer may temporarily adjust product specifications, prices, inventory, parameters and other information without notice, and there are differences in each user's consultation time and procurement needs, the platform reply content is only of reference value within a certain period. Sorry for the inconvenience caused to you, and thank you for your understanding and support! Please consult the online customer service for the latest details. Everything is subject to the real-time reply of the customer service.!

After Sales Services

Please check the quantity, appearance and product information in time when you receive the goods. In case of damage, wrong delivery, abnormal quality and other problems, please feel free to contact us.

Service Hotline:400-6226-992 ,We will handle it for you as soon as possible, thank you for your support and trust.!

Research chemical ≥95%; HPLC

Research chemical ≥95%; HPLC; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: ≥95%; HPLC.

item number:P755115-15KU
Product model:15KU
level: ≥95%; HPLC
Lead Time:30days
sold 325 Items
Ask Solution engineer
Specifications:

15KU

Qty

Recommended for you