PNGase F脑膜炎沙门氏菌

P755140-300U

300U

重组

ready-to-use solution, expressed in E. coli

重组PNGase F经亲和色谱纯化后，透析到pH 7.5、含10 mM磷酸钾的50%甘油溶液中，得到稳定的产物。该产品含有少量缓冲盐。这种高度纯化的材料可用于制备性去糖基化或用于分析凝胶、溶液或印迹膜。可利用该酶C端的6x组氨酸融合标签而将其从制备性操作中去除。来源于Elizabethkingia meningoseptica的PNGase F可用于人血浆样品的去糖基化分析和硫酸软骨素蛋白聚糖的去糖基化。用于使蛋白质去糖基化。 Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan. Used to deglycosylate protein.

来源于Elizabethkingia meningoseptica的PNGase F在N端具有聚糖结合催化结构域和碗状结构域。如果糖基化天冬酰胺部分在其氨基和羧基端被多肽链取代，则它可从糖蛋白中切割整个聚糖。 它在原核宿主中经济有效地大规模生产，需要二价锌离子才能发挥其酶促活性。从糖蛋白切割整个聚糖，前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。