细菌蛋白酶

P755344-50mg

50mg

Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

来自sigma的该酶已用于优化大鼠心脏均质化心室组织中所有线粒体群的释放。它还用于福尔马林固定石蜡包埋肝脏样本的预杂交处理，用于人和病毒DNA的检测。这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶，并在碱性条件下具有活性。 产品P8038，也被称为枯草杆菌蛋白酶Carlsberg，已用于水解心肌细胞以研究心脏线粒体NHE1的沉默。 The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA. This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis . It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1 .

蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活，如苯甲基磺酰氟（PMSF）和二异丙基氟磷酸盐。

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