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Product details
名称
碱性磷酸酶 来源于大肠杆菌
货号
P755408-25U
包装规格
25U
浓度
buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)
产品介绍
来自 大肠杆菌 的碱性磷酸酶已被用于: 荧光测定法中测定其在去磷酸化酶混合物中的酶活性 用于处理神经和 HL60 细胞裂解物中的 β-羟基 β-甲基戊二酰辅酶 A (HMG-CoA) 还原酶活性碱性磷酸酶用于偶联抗体和其他蛋白,用于 ELISA、免疫印迹和组织化学检测。需要高灵敏度时可用于蛋白标记。产品 P4069 以甘油缓冲水溶液形式提供。 Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution. Phosphatase, Alkaline from Escherichia coli has been used: in fluorometric assay to determine its enzyme activity in the dephosphorylating enzyme cocktail for dephosphorylation of peptides in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity
生化机理
碱性磷酸酶 (Alkaline phosphatase),来源于大肠杆菌,是一种主要存在于周质空间的二聚体、非糖基化蛋白质。已知存在三种亚型。这种酶需要锌,并被镁激活。大肠杆菌含皂碱磷酸酶对磷酸酯具有广泛的特异性。碱性磷酸酶是研究磷酸酯酶的模型酶。用于各种生化方法和酶联免疫吸附试验 (ELISA)。
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Research chemical

Research chemical; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number:P755408-25U
Product model:25U
level: buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)
Lead Time:30days
sold 772 Items
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25U

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