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名称

碱性磷酸酶来源于牛肠粘膜

货号

P755447-50KU

包装规格

50KU

浓度

buffered aqueous glycerol solution,≥5,500 DEA units/mg protein

产品介绍

推荐用于抗体和蛋白质缀合的高比活性等级。碱性磷酸酶可用于酪蛋白和其他蛋白质的去磷酸化。碱性磷酸酶还可用于 DNA 或 RNA 的 5′-末端的去磷酸化，以防止自我连接。经碱性磷酸酶的去磷酸化作用后，DNA 或 RNA 还可被放射性标记磷酸盐标记（通过 T4 多核苷酸激酶）。 Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. High specific activity grade recommended for antibody and protein conjugation.

生化机理

该酶对醇类、胺类、焦磷酸盐以及酚类的磷酸酯具有广泛的特异性。常用于蛋白质和核酸的去磷酸化。

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

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