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名称
碱性磷酸酯酶 来源于小牛肠
货号
P755480-250U
包装规格
250U
浓度
ammonium sulfate suspension
产品介绍
小牛肠道碱性磷酸酶(CIAP)已用于通过分光光度法评估丹磺酰基-L-苯丙氨酸的抑制作用。 CIAP还被证明能够清除脂多糖(LPS)介导的炎症反应,这在治疗LPS介导的疾病中可能具有重要作用。 来自小牛肠的碱性磷酸酶CIP可去除DNA的5′末端的磷酸基团,有助于克隆技术,防止DNA自连接。它可以被高精氨酸抑制。应用: 来自小牛肠的碱性磷酸酶是一种糖蛋白,分子量为140,000。 Alkaline phosphatase from calf intestine is a glycoprotein with a molecular weight of 140,000. Application: Alkaline phosphatase from calf intestine, CIP, removes phosphate groups from the 5′ ends of DNA which aids in cloning techniques to keep DNA from self-ligation. It can be inhibited by homoarginine. Calf intestinal alkaline phosphatase (CIAP) has been used in a study with the spectrophotometric method to assess the inhibition effect of dansyl-L-phenylalanine. It has also been shown that (CIAP) is able to detoxify the lipopolysaccharide (LPS)-mediated inflammatory response, which could be important in the treatment of LPS-mediated diseases.
生化机理
Sigma不溶性酶是通过使用常规“可溶”酶与惰性基质反应,获得保留原始酶活性的不溶性化合物而生成的。应用几乎不受限制。通过仔细选择“惰性”基质,可产生高活性的“树脂”或“凝胶”,它们与底物在合适介质中的瞬时接触,能够迅速催化酶反应。反应可以在间歇桨液反应器中进行,甚至可以在小柱中进行。无论是哪种情况,不溶性酶都不会污染反应混合物,可以很容易地将其从间歇桨液中滤出或留在色谱柱中。许多不稳定酶在不溶形式下可能会更加稳定。
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Research chemical

Research chemical; supplied for laboratory research, analysis, inspection, and scientific procurement use.

item number:P755480-250U
Product model:250U
level: ammonium sulfate suspension
Lead Time:30days
sold 628 Items
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250U

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