木瓜蛋白醇

P755552-10ml

10ml

from Carica papaya

木瓜蛋白酶可用于蛋白质的完全蛋白水解切割、天然免疫球蛋白的有限水解以及组织解离（与胶原酶、酯酶和胰蛋白酶一起使用）。该酶可溶解内在膜蛋白并水解纯蛋白多糖生成糖肽。添加半胱氨酸（约0.5% [w/v]）对酶活性至关重要。木瓜蛋白酶已用于消化各种脑细胞。应用： 木瓜蛋白酶是一种半胱氨酸蛋白酶，属于木瓜蛋白酶家族。它是单链蛋白质。木瓜蛋白酶分子量为23 kDa。木瓜蛋白酶由两个结构域和两个结构域之间的裂隙组成，这些裂隙包含活性位点。 Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site. Application: Papain has been used in the digestion of various brain cells. Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

木瓜蛋白酶对酰胺链、氨基酸酯、多肽、蛋白质具有水解活性。它主要用于切割在苯丙氨酸之后含有赖氨酸、精氨酸和残基等氨基酸的肽键。 可以催化多种反应，例如水解、转移酶作用、特异性和酰基酶中间体等。